Journal: Immunology

Article Title: The role of endothelial cell adhesion molecules P-selectin, E-selectin and intercellular adhesion molecule-1 in leucocyte recruitment induced by exogenous methylglyoxal

doi: 10.1111/j.1365-2567.2012.03608.x

Figure Lengend Snippet: Immunohistochemistry staining of P-selectin in cremasteric endothelium after methylglyoxal (MG) treatment. MG at 25 or 50 mg/kg (dissolved in 200 μl saline) was injected intrascrotally for 5·5 hr, and the mouse cremaster muscles were collected and processed as described in the Materials and methods. The brown staining on the endothelial cells of cremasteric postcapillary venules reveals the expression of P-selectin. Saline and 500 ng tumour necrosis factor-α (TNF-α) were used as negative and positive controls, respectively (magnification: 400×).

Article Snippet: EA.hy926 cell, a hybrid human umbilical vein endothelial cell line, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Immunohistochemistry, Staining, Saline, Injection, Muscles, Expressing

Journal: Immunology

Article Title: The role of endothelial cell adhesion molecules P-selectin, E-selectin and intercellular adhesion molecule-1 in leucocyte recruitment induced by exogenous methylglyoxal

doi: 10.1111/j.1365-2567.2012.03608.x

Figure Lengend Snippet: Immunohistochemistry staining of E-selectin on cremasteric endothelium after methylglyoxal (MG) treatment. MG at 25 or 50 mg/kg (dissolved in 200 μl saline) was injected intrascrotally for 5·5 hr, and the mouse cremaster muscles were collected and processed as described in the Materials and methods. The brown staining on the endothelial cells of cremasteric postcapillary venules reveals the expression of E-selectin. Saline and 500 ng tumour necrosis factor-α (TNF-α) were used as negative and positive controls, respectively (magnification: 400×).

Article Snippet: EA.hy926 cell, a hybrid human umbilical vein endothelial cell line, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Immunohistochemistry, Staining, Saline, Injection, Muscles, Expressing

Journal: Immunology

Article Title: The role of endothelial cell adhesion molecules P-selectin, E-selectin and intercellular adhesion molecule-1 in leucocyte recruitment induced by exogenous methylglyoxal

doi: 10.1111/j.1365-2567.2012.03608.x

Figure Lengend Snippet: Immunohistochemistry staining of intracellular adhesion molecule 1 (ICAM-1) on cremasteric endothelium after methylglyoxal (MG) treatment. MG at 25 or 50 mg/kg (dissolved in 200 μl saline) was injected intrascrotally for 5·5 hr, and the mouse cremaster muscles were collected and processed as described in the Materials and methods. The brown staining on the endothelial cells of cremasteric postcapillary venules reveals the expression of ICAM-1. Saline and 500 ng tumour necrosis factor-α (TNF-α) were used as negative and positive controls, respectively (magnification: 400×).

Article Snippet: EA.hy926 cell, a hybrid human umbilical vein endothelial cell line, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Immunohistochemistry, Staining, Saline, Injection, Muscles, Expressing

Journal: Immunology

Article Title: The role of endothelial cell adhesion molecules P-selectin, E-selectin and intercellular adhesion molecule-1 in leucocyte recruitment induced by exogenous methylglyoxal

doi: 10.1111/j.1365-2567.2012.03608.x

Figure Lengend Snippet: Immunohistochemistry staining of vascular cell adhesion molecule 1 (VCAM-1) on cremasteric endothelium after methylglyoxal (MG) treatment. MG at 25 or 50 mg/kg (dissolved in 200 μl saline) was injected intrascrotally for 5·5 hr, and the mouse cremaster muscles were collected and processed as described in the Materials and methods. Saline and 500 ng tumour necrosis factor-α (TNF-α) were used as negative and positive controls, respectively. The brown staining on the endothelial cells of cremasteric postcapillary venules reveals the expression of VCAM-1 in the TNF-α-treated group. Both MG treatment groups show no apparent change of VCAM-1 expression after 5·5 hr MG treatment compared with saline control group (magnification: 400×).

Article Snippet: EA.hy926 cell, a hybrid human umbilical vein endothelial cell line, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Immunohistochemistry, Staining, Saline, Injection, Muscles, Expressing, Control

Journal: Immunology

Article Title: The role of endothelial cell adhesion molecules P-selectin, E-selectin and intercellular adhesion molecule-1 in leucocyte recruitment induced by exogenous methylglyoxal

doi: 10.1111/j.1365-2567.2012.03608.x

Figure Lengend Snippet: Methylglyoxal (MG)-induced expression of P-selectin, E-selectin and intracellular adhesion molecule 1 (ICAM-1) in cultured endothelial cells. EA.hy926 endothelial cells were cultured to 90% confluence and treated with PBS (negative control), 100 μm MG or 20 ng/ml tumour necrosis factor-α (TNF-α; positive control) for 4 hr. The expression of P-selectin, E-selectin and ICAM-1 in cell lysates was determined by Western blot.

Article Snippet: EA.hy926 cell, a hybrid human umbilical vein endothelial cell line, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Expressing, Cell Culture, Negative Control, Positive Control, Western Blot

Journal: Immunology

Article Title: The role of endothelial cell adhesion molecules P-selectin, E-selectin and intercellular adhesion molecule-1 in leucocyte recruitment induced by exogenous methylglyoxal

doi: 10.1111/j.1365-2567.2012.03608.x

Figure Lengend Snippet: Effect of nuclear factor-κB (NF-κB) inhibition on methylglyoxal (MG) -induced endothelial adhesion molecule expression and leucocyte recruitment. BAY11-7082 at 20 mg/kg was injected to the mice 30 min before 25 mg/kg MG administration. The vehicle control group was injected with the same concentration of the solvent for BAY11-7082. After 4-hr MG injection, the mouse cremaster muscle was prepared for intravital microscopy. The muscle samples were then collected and processed for immunostaining after 1·5 hr intravital microscopy. The brown staining on the endothelial cells of cremasteric postcapillary venules reveals the expression levels of P-selectin, E-selectin and intracellular adhesion molecule 1 (ICAM-1; magnification: 400×) in the upper, middle and lower panels, respectively (a). The inhibitory effects of BAY11-7082 on MG-induced leucocyte rolling flux (b), rolling velocity (c), the number of adherent leucocytes (d) and the number of emigrated leucocytes (e) were determined. Values in (b) to (e) are means ± SEM (n = 3). *P < 0·05 compared with 25 mg/kg MG treatment group without BAY 11-7082.

Article Snippet: EA.hy926 cell, a hybrid human umbilical vein endothelial cell line, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Inhibition, Expressing, Injection, Control, Concentration Assay, Solvent, Intravital Microscopy, Immunostaining, Staining